Phase Holographic Image

HoloMonitor— A Cell Culture Microscope

The inverted HoloMonitor cell culture microscope facilitates label-free single-cell analysis on a population level in real-time. HoloMonitor operates within a CO2 incubator or hypoxia chamber, enabling long-term studies of in vitro cell popu­lations in a stable and physio­logi­cally relevant environment. HoloMonitor non-invasively quantifies more than 30 cellular parameters reflecting:

  • Cell proliferation

  • Cell migration and invasion

Cell morphology and differentiation

 

CELL-FRIENDLY LONG-TERM ANALYSIS

From just a single cell culture experiment you can learn how treatments affect the response and behavior of your cell culture samples. HoloMonitor is an incubator tolerant phase imaging microscope. This allows HoloMonitor to provide label-free cell culture analysis in an optimal environment over extended periods of time.

SINGLE-CELL ANALYSIS ON A POPULATION LEVEL

HoloMonitor deploys quantitative phase contrast microscopy to non-invasively analyze cellular events by long-term digital phase imaging. While recording, the time-lapse image sequences are automatically analyzed to in real-time provide single-cell and population information in the form of graphs and scatterplots.

FIRST POINT OF INVESTIGATION

Contrary to fluorescence microscopy, the cells are non-invasively imaged with a low power laser without labels or stains. This leaves the cells completely unaffected for further down­stream studies using complementary instru­mentation, for example, fluorescent imaging by high-resolution confocal microscopy.

 

phiab.com

 

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