qPCR Probes

Dual-Labeled BHQ Probes:

Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences.

BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference.


BHQplus Probes:

The BHQplus® probe is a novel compact probe for qPCR, modified with a duplex stabilizing technology and terminated with Biosearch’s Black Hole Quencher® dye. What differentiates the BHQplus probe from other traditional probes is that it permits the design of shorter oligonucleotides, typically 15 - 25 bases in length, for detecting more difficult target sequences. The enhanced specificity of BHQplus probes make them perfect for genotyping single nucleotide polymorphisms.

BHQplus probes are offered in two convenient deliveries. For BHQplus probes and primers delivered in individual tubes select just “BHQplus probes”, however if you would like the probes and primers blended into a single tube select “ValuMix Assay for SNP genotyping”.

ValuMix™ Assays for SNP genotyping comprises two custom BHQplus® probes and two primers combined within a single tube. This formulation minimizes pipetting error and simplifies your protocol by providing mixed oligonucleotides ready for SNP genotyping.