Zymo Cloning

The Mix & Go E. coli Transformation Kit and Mix & Go E. coli Transformation Buffer Set are convenient methods for the preparation of competent E. coli cells for simple and highly efficient DNA transformation. The Mix & Go method completely eliminates the requirement for heat shocking and related procedures. Instead, Mix & Go bacterial transformation can be performed by adding DNA to Mix & Go competent cells and spreading onto a plate. Transformation efficiencies are typically on the order of 108-109 transformants/µg plasmid DNA with most E. coli strains.